Genotoxicity of Human Mammary Lipid1
نویسندگان
چکیده
We tested the proposition that human mammary lipid contains muta genic/genotoxic agents that could cause DNA damage in adjacent epithe. hal cells. Lipid samples from breast tissue surgically removed from 40 women undergoing elective reduction mammoplasty were extracted by a solid-phase procedure. Mutagenicity was observed in Salmonella typhi murium TA98 and TA1538 in 16 of 40 (40%) extracts assayed with rat-liver S9, but not in its absence. No mutagenicity was seen in S. typhimurium TA100 or Escherichia coli WP2uvrA(pKM1O1). Bacterial mutagenicity correlated with micronucleus-forming activity in a metabol ically competent mammalian cell line (MCL-5). This genotoxic activity merits further investigation in relation to the etiology of breast cancer.
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